Proteoglycans are cell surface linked macromolecules which through protein modules and glycan moieties modulate numerous cellular interactions during embryogenesis, healthy and pathologic state, including tumor progression and neoangiogenesis (1).

PRODUCT

1. Each vial contains the indicated amount of IgG (caprylic acid purified) in 0.1% gelatine and 0.05% NaN3.

2. Unpurified reagent is provided at the indicated amounts with 0.1% NaN3.

Centrifuge the vial prior to use.

SPECIFICTY

The murine monoclonal antibody MP-AA-8 (IgG 1) recognizes a cell surface condroitin sulphate proteoglycan, the human homologue of the rat NG2. Firstly described in melanoma cells (2,3), is also expressed by a fraction of human gliomas and soft-tissue sarcomas (fibrosarcomas, fibrohistiocytoma). While not expressed by normal hematopoietic cells, it is expressed in childhood and adult AML and ALL with MLL gene rearrangement (4). It is a highly specific biomarker of desmoplastic melanoma (5).

STORAGE

Store at 4°C, avoid repeated freezing-thawing. Stable for 2 yrs.

SHIPPING CONDITIONS

Room temperature.

RESEARCH USE

This antibody is for laboratory research use only, not for human or in-vivo use.

APPLICATIONS

Flow cytometry, 5 µ/ml.

Immunohistochemistry (fluorescence or enzymatic), Acetone fixed cryostat sections and cytospins (10 µg/ml).

Immunohistochemistry on formalin-fixed, paraffin-embedded tissue: 5 microns deparaffinized sections are exposed at MW at 750W, in EDTA buffer pH.8.0 for 15 min. Antibody is incubated with sections at concentration of 20-30 ug/ml overnight. Immune reaction is detected with any immunoenzymatic kit following the producer’s instructions

Immunoprecipitation, 5-10 µg, using rabbit anti-mouse Ig and protein- A Sepharose beads.

REFERENCES
1. Macromolecular Bioscience (2006) 6:667-680.
2. Int J Cancer (1987) 39:729-736.
3. Int J Cancer (2003) 103:399-407.
4. Leukemia (2003) 17:1589-1595.
5. Pigm. Cell Melan. Res. (2009) 23:137-140.